ABC Transporters
Membranes for vesicular transport assays

SOLVO Biotechnology offers membrane vesicles prepared from Sf9 (Spodoptera frugiperda ovarian) cells or mammalian cell lines. The membrane vesicles contain a wide range of different ABC transporters and can be utilized in different assays. SOLVO also offers control vesicles with no, or significantly lower transporter expression both for studies on Sf9 membrane preparations or mammalian membranes.


The product package includes:

  • Isolated membranes, containing the desired transporter proteins (3-5% of total membrane proteins). Aliquoted in 5 mg membrane protein/ml suspensions, in 2.5 mg (500 μl) units.
  • Detailed assay protocols for the Vesicular Transport assay.
  • Individual signed datasheets.

Transporter (gene) Catalogue # Intended use Reference substrate ATP dependent transport*
MRP1 (ABCC1) SB-MRP1-Sf9-VT Vesicular transport 3H-LTC4
20-45
MRP2 (ABCC2) SB-MRP2-Sf9-VT Vesicular transport 3H-E2-17βG
300-800
Vesicular transport 3H-LTC4
 6-20
MRP3 (ABCC3) SB-MRP3-Sf9-VT Vesicular transport 3H-E2-17βG
 20-35
MRP5 (ABCC5) SB-MRP5-HEK293-VT Vesicular transport 3H-cGMP
 0.4-0.7
BCRP / MXR (ABCG2) SB-BCRP-Sf9-VT Vesicular transport 3H-Methotrexate 35-65
BCRP / MXR (ABCG2) SB-BCRP-M-VT Vesicular transport 3H-Estrone-3-sulfate
 40-100
Vesicular transport 3H-Methotrexate
40-90
BSEP (ABCB11) SB-BSEP-Sf9-VT Vesicular transport 3H-Taurocholate
15-35
mouse BSEP SB-mouseBsep-Sf9-VT Vesicular transport 3H-Taurocholate
100-400
defMRP SB-defMRP-Sf9-CTRL Control for MRP1-, MRP2-, MRP3- and BSEP-Sf9 Vesicular transport assay 3H-LTC4 <6
3H-E2-17βG
<50
3H-E2-17βG
<2
3H-Taurocholate
<5
defBCRP SB-defBCRP-Sf9-CTRL Control for BCRP-Sf9 Vesicular transport assay 3H-Methotrexate
<5
M SB-M-CTRL Control for BCRP-M Vesicular transport assay 3H-Estrone-3-sulfate
<5
3H-Methotrexate
<5
HEK293 SB-HEK293-CTRL Controll for MRP5 Vesicular transport assay 3H-cGMP
0.05-0.15
                                                                                                                                                                                *[pmol /mg/min]