SOLVO Biotechnology offers membrane vesicles prepared from Sf9 (Spodoptera frugiperda ovarian) cells or mammalian cell lines. The membrane vesicles contain a wide range of different ABC transporters and can be utilized in different assays. SOLVO also offers control vesicles with no, or significantly lower transporter expression both for studies on Sf9 membrane preparations or mammalian membranes.

The product package includes:

• Isolated membranes, containing the desired transporter proteins (3-5% of total membrane proteins). Aliquoted in 5 mg membrane protein/ml suspensions, in 2.5 mg (500 μl) units.
• Detailed assay protocols for the ATPase assay (HTS where available).
• Individual signed datasheets.

Transporter (gene)	Catalogue #	Intended use	Reference substrate	fold activation
MDR1 / PgP (ABCB1)	SB-MDR1-Sf9-ATPase	ATPase activation	Verapamil (40 μM)	3-5
		ATPase inhibition	Verapamil (40 μM)
rat Mdr1b	SB-ratMdr1b-Sf9-ATPase	ATPase activation	Verapamil (200 μM)	4-8
		ATPase inhibition	Verapamil (200 μM)
MRP1 (ABCC1)	SB-MRP1-Sf9-ATPase	ATPase activation	NEM-GS (10 mM)	2-3
		ATPase inhibition	NEM-GS (10 mM)
MRP2 (ABCC2)	SB-MRP2-Sf9-ATPase	ATPase activation	Probenecid (1 mM)	2.5-4
		ATPase inhibition	Probenecid (1 mM)
rat Mrp2	SB-ratMrp2-Sf9-ATPase	ATPase activation	Probenecid (4 mM)	2-4
		ATPase inhibition	Probenecid (4 mM)
MRP3 (ABCC3)	SB-MRP3-Sf9-ATPase	ATPase activation	Benzbromarone (50 µM)	2-4
		ATPase inhibition	Benzbromarone (50 µM)
BCRP / MXR (ABCG2)	SB-BCRP-HAM-Sf9-ATPase	ATPase activation	Sulfasalazine (10 µM)	2-4
		ATPase inhibition	Hoechst 33342 (10 µM) / KO143 (100 nM)	0-1
BCRP / MXR (ABCG2)	SB-BCRP-M-ATPase	ATPase activation	Sulfasalazine (10 µM)	2-4
		ATPase inhibition	Hoechst 33342 (10 µM) / KO143 (100 nM)	0-1
BCRP / MXR-R482G (ABCG2-R482G)4	SB-BCRP-R482G-Sf9-ATPase	ATPase activation	Prazosine (50 µM)	1.5-2
		ATPase inhibition	Hoechst 33342 (10 µM) / KO143 (100 nM)	0-1
defMRP	SB-defMRP-Sf9-CTRL	Control for MDR1-, ratMdr1b-, MRP1-, MRP2-, ratMrp2-, MRP3-Sf9 ATPase assay	See above MDR1, MRPs, ratMdr1b and ratMrp2 reference substrates	-
defBCRP	SB-defBCRP-Sf9-CTRL	Control for BCRP-Sf9 ATPase assay	See above SB-BCRP-Sf9 reference substrates	-
M	SB-M-CTRL	Control for BCRP-M ATPase assay	See above SB-BCRP-M reference substrates	-