The Extracel Hydrogel-24 and -96 offer the advantages of Glycosan’s Extracel formula in a convenient, ready to use hydrogel. To use, simply open them under aseptic conditions and add your cells. Extracel Hydrogels are ideal for culturing primary cells and cell lines on a compliant hydrogel surface that readily promotes cell attachment^1,2. Unlike animal-derived extracellular matrices (ECM), Extracel Hydrogels are chemically fully defined and consistent from lot to lot. They are based on three biocompatible components: thiol-modified hyaluronan (a major constituent of native ECM), thiol-modified gelatin (denatured collagen), and a thiol-reactive crosslinker, polyethylene glycol diacrylate.

Formats

• The Extracel Hydrogel-24 comes in a 24-well plate pre-filled with ready to use hydrogels that have a volume of 300 µl.
• The Extracel Hydrogel-96 comes in a 96-well plate pre-filled with ready to use hydrogels that have a volume of 100 µl.

Hydrogel Rigidity

The Extracel Hydrogels are designed to have a compliance of 100-500 Pa.

References

1. X.Z. Shu, S. Ahmad, Y. Liu, G.D. Prestwich, “Synthesis and evaluation of injectable, in situ crosslinkable synthetic extracellular matrices for tissue engineering,” J Biomed Mater Res A, 79A(4), 901-912 (2006).
2. K. Ghosh, Z. Pan, E. Guan, S. Ge, Y. Liu, T. Nakamura, X. Ren, M. Rafailovich, R. Clark, “Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties,” Biomaterials 28, 671–679 (2007).