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PrintPhosphodiesterases
The BIOMOL Cyclic Nucleotide Phosphodiesterase (PDE) Assay Kit is a colorimetric, non-radioactive assay designed in a microplate format. It may be used to
screen inhibitors and modulators of cyclic nucleotide phosphodiesterase activity.
The assay offers the following advantages:
The basis for the assay is the cleavage of cAMP or cGMP by a cyclic nucleotide phosphodiesterase. The 5’-nucleotide released is further cleaved into the
nucleoside and phosphate by the enzyme 5’-nucleotidase. The phosphate released due to enzymatic cleavage is quantified using BIOMOL GREENTM reagent
in a modified Malachite Green assay. The kit includes Type I cyclic AMP phosphodiesterase (PDE) for validation purposes.
A nonspecific cyclic nucleotide phosphodiesterase inhibitor, 3- isobutyl-1-methylxanthine (IBMX) is included as a test control for inhibitor screening. It has an IC50 of approximately 25 μM.
Individual components of this kit are available separately
screen inhibitors and modulators of cyclic nucleotide phosphodiesterase activity.
The assay offers the following advantages:
- NON-RADIOACTIVE
- CONVENIENT ONE STEP DETECTION
- MICROPLATE FORMAT
The basis for the assay is the cleavage of cAMP or cGMP by a cyclic nucleotide phosphodiesterase. The 5’-nucleotide released is further cleaved into the
nucleoside and phosphate by the enzyme 5’-nucleotidase. The phosphate released due to enzymatic cleavage is quantified using BIOMOL GREENTM reagent
in a modified Malachite Green assay. The kit includes Type I cyclic AMP phosphodiesterase (PDE) for validation purposes.
A nonspecific cyclic nucleotide phosphodiesterase inhibitor, 3- isobutyl-1-methylxanthine (IBMX) is included as a test control for inhibitor screening. It has an IC50 of approximately 25 μM.
Individual components of this kit are available separately
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