Our partner Cytoskeleton Inc provides the widest range available of kits to assay kinesins. These assays include kinetic and endpoint ATPase assays for kinesins as well as kinesin motility assays and microtubule depolymerization assays for depolymerizing kinesins such as MCAK.

Kinesin HTS ATPase EndPoint assay: Cat nr BK053




Kinesin ELIPA^TM Biochem Kit^TM: Cat nr BK060

IC50 determination of Monastrol inhibition of kinesin motor activity. Kinesin motors at 1.0, 1.0 and 0.2 μM (BimC, Eg5 and hKHC respectively) were mixed with 4 μM tubulin in the form of microtubules (Cat. # MT001). Reactions were initiated with the addition of ATP at 500 μM. Colorimetric readings were reduced to Vmax values, which were used to plot the dose-response curves shown above. Further details of the assay process can be obtained from the manual (available on request) or as described in Funk et al. 2004, Anal. Biochem., 329,68-76.