The ArrayPure™ Nano-scale RNA Purification Kit (Epicentre, Madison, WI)provides all of the reagents needed to purify RNA from ten to a few thousand eukaryotic cells, such as obtained from Laser Capture Microdissection (LCM) procedures. The reagents are all aqueous to avoid the use of toxic organic solvents. This nano-scale protocol has been developed and tested with quantitative real-time PCR on 10 to 10,000 eukaryotic cells. Cell numbers were based on living cells that were enumerated and then diluted. The average yields of RNA from 1,000 and 10,000 HeLa cells, as determined by RiboGreen® fluorescence, were 21 and 213 ng, respectively. In addition, µg amounts of RNA have been produced from 20 HeLa cells using aRNA 2-round synthesis techniques.

Applications:

- RT-PCR
- aRNA
- LCM

Benefits:

- Quantitatively isolate RNA from as few as 10 cells
- Purify RNA for aRNA synthesis
- Simple, nontoxic procedure