Purification & Recovery of Nucleic Acids
Fosmid DNA
The FosmidMAX™ DNA Purification Kit (Epicentre, Madison, WI) was developed for easy, reliable isolation of high-quality fosmid DNA. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 µg of fosmid DNA from 1.5–100 ml of a single-copy fosmid culture.

Applications: Isolate exceptionally pure fosmid DNA that can be used for many applications including sequencing, fingerprinting, PCR and preparation of shotgun libraries

Benefits:

- Superior yields —Easy to follow protocols for isolating up to 0.6, 15, or 25 µg of fosmid DNA from 1.5, 40 or 100 ml cultures of a single-copy fosmid, respectively
- Convenient —Eliminate the need for columns, resins, or organic extractions.
Consistent results
- Versatile —Ideal for isolating DNA from single-copy fosmid clones, induced CopyControl™ fosmid clones and cosmids.