The MasterPure™ Plant Leaf DNA Purification Kit (Epicentre, Madison, WI)is designed for isolating DNA from 35-100 mg of fresh plant leaf tissue. The protocol can be completed in less than one hour and may be scaled up to obtain larger amounts of DNA.

The MasterPure Kit was designed for even the most difficult plant leaf DNA purifications. The kit was optimized with the grapevine, Vitis vinifera, whose leaves are notoriously high in polyphenolic (tannin-like) compounds and polysaccharides, which can interfere in both the isolation and subsequent utilization of DNA. No enzymatic digestions, CTAB (hexadecyltrimethylammonium bromide), or organic solvents are used in the MasterPure protocol.

Applications:

- Identification of plant species by microsatellite typing
- PCR amplification, restriction digestion, and Southern blotting

Plants tested:

grape, apple, tomato, corn, sugarcane, sunflower, fern, quillwort, pine

Benefits

- Versatile—recover DNA from the leaves of many plant species, even those high in polysaccharides and polyphenolic compounds
- Rapid—purify DNA in less than one hour.
- Yields high molecular weight DNA
- Simple and non-toxic—eliminates organic solvent and CTAB extractions, CsCl gradients, and enzyme digestions
- Recover DNA of higher integrity than column-based purification methods