The MasterPure™ Yeast DNA Purification Kit (Epicentre, Madison, WI) enables efficient, high-yield purification of high molecular weight DNA from yeast and other fungi. The protocol involves non-enzymatic cell lysis at 65°C, followed by removal of protein by precipitation, then nucleic acid precipitation and resuspension . No lyticase or proteolytic enzymes are used in the procedure.

Yeast genomic DNA yields using the MasterPure Kit are much higher than yields obtained with other commercially available kits (Figure 1).1 The protocol can be easily adjusted for larger or smaller samples, including single yeast colonies.1 The recovered nucleic acid can be used directly in many applications including PCR amplification. The MasterPure Yeast DNA Purification Kit is available in two sizes, either 10 or 200 DNA purifications from 1.5 ml of yeast culture.

Applications

- PCR amplification
- restriction endonuclease digestion
- Southern blotting
- genomic library preparation.
- Identification and typing of fungi

Benefits

- Higher yields of yeast chromosomal DNA
- Versatile—recover DNA from a wide variety of yeast species including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus2 and Penicillium
- Rapid—purify DNA in less than 40 minutes
- Simple and safe—eliminates the need for enzymatic lysis, columns, and phenol or other organic extractions