The MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Madison, WI) provides all of the reagents needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse™ and the Gram Positive Cell Lysis Solution. Ready-Lyse™ Lysozyme is a stable solution of a non-mammalian, non-avian recombinant lysozyme, with high specific activity and no net charge at neutral pH. Thus, there is no waiting to dissolve the lysozyme and it does not bind DNA.1,2

The variety of gram positive bacteria tested with this Kit is:
- Bacillus subtilis
- Listeria monocytogenes
- Staphylococcus aureus
- Streptococcus mutans
- Lactococcus lactis


Applications: Obtain genomic DNA from gram positive bacteria for

- fosmid library construction
- PCR
- restriction digests
- Southern blotting.

Benefits

- PCR-ready DNA
- Scalable method
- High molecular weight DNA
- No extra enzyme to purchase
- Tested on Bacillus, Streptococcus and Listeria