The SoilMaster™ DNA Extraction Kit (Epicentre, Madison, WI) provides all the reagents necessary to recover PCR-ready DNA from a variety of environmental samples. The kit utilizes a hot detergent lysis process combined with a simple chromatography step that removes organic inhibitors, such as humic and fulvic acids, that are known to co-extract with DNA from soil and sediment samples.

The SoilMaster™ Kit isolates larger and more intact DNA than other soil DNA kits incorporating bead beating or vortex mixing in the presence of beads. The extracted DNA is PCR-ready, and can be used to amplify bacterial, plant, or fungal templates .

Applications:
- Extraction of PCR-ready DNA from soil and sediment for PCR amplification of
diverse organisms
- Humic acid and protein removal

Benefits:
- Rapid—extract PCR-ready soil DNA in less than 45 minutes
- Simple—no need for dedicated equipment or bead beating
- Larger, higher molecular weight DNA recovery