Service: Molecular Biology

You can save time and effort by outsourcing your Molecular Biology project to tebu-bio. We will define with you the best customized strategy to fit your requirements.

    Modularity Each project is divided into modules and go/no go steps for more flexibility & security. Customisation Fully customised projects to match your requirements.
    Follow-upA dedicated project manager for regular updates on your project.

    Confidentiality Strict confidentiality rules.
    Quality Controls & Standard Operating Procedures (SOPs)  

Cloning

We can help you in your cloning strategy, whether we start from your ready-to-use insert or whether we help you customize your vector construction for protein expression…

  • Insert: cDNA, PCR product, oligos/adapters, microDNA or RNA
  • Vector: any plasmid supplied by the researcher

Basic cloning steps:

  • DNA restriction & purification
  • Ligation & E.coli transformation
  • Screening (minipreps or PCR)
  • Restriction profile & maxiprep

Customized vector construction:

  • Basic cloning steps
  • Insert engineering by PCR amplification of supplied cDNAs (tags, endopeptidases, signal peptide…), multiple cloning steps.
  • Quality control: double-strand sequencing of full insert

Directed Mutagenesis

  • Site directed mutagenesis (point mutation, insertion, deletion)
  • PCR amplification of full vector with mutant primers (QC by double-strand sequencing of mutated insert.)
  • DNA restrictions/sub cloning of modified insert in original vector
  • Screening (minipreps or PCR), Restriction

Plasmid prep lab services

  • Low endotoxin conditions Ready for in-vivo use
  • Scalable volumes (from 200 ml to 5 litres culture) to suit your needs (up to 100 mg of plasmid)
  • Plasmid archiving service Glycerol stocks are stored for up to 2 years for immediate availability of your constructs
standard Price for 4 plasmids preparation Price in €
Standard Offer  
MaxPrep 800.00
Giga Prep 950.00
Optional Services  
Bacterial transformation (EC100™ bacterial strain**) 300.00
Pre-extraction control (DNA mini-preparations, restriction profile analysis) 400.00
Post-extraction control (OD260/280 readings and restriction profile analysis) 300.00
Endotoxin level measure 400.00

* All prices are excluding VAT and FOB.
** From Epicentre ® (Madison, WI)

Please fill our form or contact Nathalie Bervas to discuss your project.