The Cignal Reporter Assay System (SABiosciences, frederick, MD) provides a comprehensive solution for monitoring diverse cell signaling pathways. These reporter assays are valuable tools for assessing the biological impact of RNA interference, protein overexpression or small molecule drug treatment. Cignal Reporter Assays enable you to rapidly identify which cell signaling pathways contribute to the function of your favorite genes or chemical compounds.

These assays provide a rapide, sensitive and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors, using either dual-luciferase or green fluorescent protein (GFP) reporter systems.
 

Why Cignal Reporter Assays?

• Reproducibility
  Functionally validated dual reporter formulation minimizes variability, increasing the biological relevance of each experiment.
• Versatility and Sensitivity
  Proprietary TRE structure allows monitoring both up- and down-regulations. Use of destabilized luciferase increases the signal to noise ratio, maximizing assay sensitivity.
• Convenience
  Transfection-ready easy-to-use assay kits, including positive and negative controls, coupled with transient reporter system, enable rapid analysis of signal transduction pathway regulation.

Single Cignal Reporter Assays to monitor individual pathway

• The dual-luciferase format is an endpoint assay providing unsurpassed sensitivity, specificity, and signal-to-noise ratios. This format is available for all 18 Cignal Reporter Assays. Each assay includes a pathway-focused transcription factor reporter, a non-inducible negative control, as well as luciferase and GFP positive controls.

• The GFP format is an outstanding method for monitoring live cell pathway regulation, with single cell resolution. This format is currently available for six of the Cignal Reporter Assays. It includes the pathway-focused transcription factor reporter, and the necessary negative and positive controls.

10-pathways Cignal Reporter Arrays to obtain a conprehensive view of multiple pathways involved in a biological process

In a single experiment you can use the Cignal Finder 10-Pathway Reporter Arrays to simultaneously measure the activities of 10 cell signaling pathways known to play critical roles in the biological process being studied in your laboratory. Don't rely on guesswork to predict mechanisms of action. Use the Cignal Finder Arrays to definitively discover important gene functions and pathway interactions.