One band after IP assay
The ExactaCruzTM technology is designed to detect your WB primary antibody against the protein of interest without detection of heavy and light chains of the IP antibody.
This sytem is ideal for analysis of cellular proteins expressed at very low levels.

The ExactaCruz™ product line provides a new and improved method for the detection of immunoprecipitated proteins via Western Blot (WB) analysis 5santa Cruz Biotechnology).

When used as directed, ExactaCruz™ effectively eliminates the detection of heavy and light chains of the IP antibody. We provides six unique detection systems that apply to IP/WB that use any combination of goat, rabbit and mouse antibodies as the IP and Western Blotting (WB) antibodies.

Each kit contains the required IP matrix to precipitate the desired Ag-Ab complex and an HRP conjugated reagent that detects only the desired WB antibody. ExactaCruz™ technology is of particular value for the analysis of cellular proteins that are expressed at very low levels and thus difficult to detect using conventional Western Blotting procedures.

Please find below references of ExactaCruzTM suitable to your experiments:

PRODUCT NAME CAT nr
USE
SPECIES OF WB
ANTIBODY
SPECIES OF IP
ANTIBODY
ExactaCruzTM A sc-45038 heterologous IP/WB goat rabbit or mouse
ExactaCruzTM B
sc-45039 heterologous IP/WB mouse goat or rabbit
ExactaCruzTM C
sc-45040 heterologous IP/WB rabbit goat or mouse
ExactaCruzTM D
sc-45041 homologous IP/WB goat goat
ExactaCruzTM E
sc-45042 homologous IP/WB mouse mouse
ExactaCruzTM F
sc-45043 homologous IP/WB rabbit rabbit