Target-specifc silenced cell lines available for:

Drug discovery	Functional studies	HTS
Target identification	Pathway detection	Assay development
Target validation	Drug screening	ADME-Tox

 

Human genome-wide SilenciX for target studies

The availability of cellular models with silenced genes offers the possibility of hands-on studies based on RNA interference (RNAi). Access to such cell lines helps the researcher focus on experimental questions without the need to first construct a cellular model. tebu-bio offers SilenciX human adherent cells that have been modified by nonviral and safe transfection for long-term silencing of genes by RNAi.

tebu-bio has developped catalogued silenced HeLa cell lines. We are continuously expanding our SilenciX list to meet your research needs. We invite your suggestions of new human target knock-down to put at our top of SilenciX production. Feel free to contact your local office or our product specialist: Aurélie SERRA, PhD.

Custom construction services using our SilenciX technology is also available in the human cell line of your choice with your gene of interest.

Each kit contains:

• 1 vial of "target specific" SilenciX cells, validated by qPCR
• 1 vial of control SilenciX cells (transfected with a non-relevant shRNA sequence)
• a complete user manual and specification sheets for each cell line.

SilenciX cell lines benefits

Guaranteed knock-down

• shRNA-based technology
• More than 70% extinction at RNA level (data shown on data sheet)

 

Stable silencing

• Long-term efficiency (>500 days) => see recurrent publications with the same cell lines

Robust cell model with no off-target effects

• New algorithm called DSIR (Designer of Small Interfering RNA) for predicting siRNA efficacy based on a simple linear model (further information)
• Best performer method among existing algorithms (Matveeva et al, 2007)
• High specificity of shRNA sequence to the target

 

Safe & Convenient

• Virus-free system
• No integration in cell genome

 

 

SilenciX last publications

PARP inhibition vs. PARP-1 silencing different outcomes in terms of single-strand break repair and radiation susceptibility
Godon et al., Cell Cycle 2008; in press

Moderate variations in CDC25B protein levels modulate the response to DNA damaging agents
Aressy et al., Nucleic Acids Res. 2008; in press

Role of ATM in the telomere response to the G-quadruplex ligand 360A
Pennarun et al., Nucleic Acids Res. 2008; 36(5):1741-54

Interplay between cernunnos-XLF and NHEJ proteins at DNA ends in the cell
Wu et al., J. Biol. Chem. 2007;282(44):31937-43

Untangling the relationships between DNA repair pathways by silencing more than 20 DNA repair genes in humn stable clones.
Biard, Nucleic Acids Res. 2007 June; 35 (11): 3535–3550

Long-term XPC silencing reduces DNA double-strand break repair
Despras et al., Cancer Res 2007 March; 67 (6): 2526–2534

For more information please contact our product specialist: Aurélie SERRA, PhD.

SilenciX is a trademark of tebu-bio.
Technology licensed from
DSIR algorithm was developped jointly by the Center for Computational Biology at the Ecole des Mines de Paris and the Laboratoire BIM (Biologie, Informatique et Mathematique) at the CEA.