The MessageBOOSTER™ Kit amplifies mRNA from total RNA of 1 - 50 cells (10 pg - 500 pg total RNA) and then produces cDNA ready for real-time qPCR.

• Enables sensitive and reproducible RT-PCR of even low abundance transcripts using total RNA from as little as 1 cell.
• Utilizes an improved and simplified “Eberwine” linear RNA amplification procedure that preserves the gene expression profile.
• Produces enough single-stranded cDNA from each reaction for up to 10 real-time PCR (qPCR) reactions of low-abundance transcripts from 1 cell.

Applications

• Quantitative real-time RT-PCR (qRT-PCR) using total RNA from 1 to 50 cells (approximately 10 pg to 500 pg of total RNA)

Benefits

• Produces cDNA for sensitive and reproducible qPCR of even low-abundance transcripts (mRNA) from total RNA of 1 to 50 cells.
• Linear RNA amplification process preserves the gene expression profile.
• Each reaction produces enough cDNA for up to 10 qPCR reactions for low-abundance transcripts from total RNA of 1 cell (approximately 10 pg total RNA).
• Enables multiplex qRT-PCR using total RNA from 1 cell.
• MMLV Reverse Transcriptase is provided in the kit and reactions are readily adapted for use with SuperScript™ III Reverse Transcriptase (provided by the user) if desired.

Amount of Total RNA in a MessageBOOSTER™ Reaction	Number of Real-Time qPCR Reactions That Can Be Performed
	Low- to Medium- Abundance Transcripts*	Medium- to High- Abundance Transcripts*
10 pg (~1 cell)	≥10	≥100
100 pg (~10 cells)	≥100	≥1,000
500 pg (~50 cells)	≥500-1,000	≥5,000-10,000
*Low-Abundance Transcripts = 1-1,000 copies per cell Medium-Abundance Transcripts = 1,000-10,000 copies per cell High-Abundance Transcripts = 10,000-100,000 copies per cell

Table 1. The Number of qPCR reactions that can be performed using cDNA produced by a MessageBOOSTER™ reaction is dependent on the amount of input total RNA and the abundance of the transcript(s) of interest.

The ArrayPure™ Nano-scale RNA Purification Kit provides all of the reagents needed to purify RNA from ten to a few thousand eukaryotic cells, such as obtained from Laser Capture Microdissection (LCM) procedures). The reagents are all aqueous to avoid the use of toxic organic solvents. This nano-scale protocol has been developed and tested with quantitative real-time PCR on 1 to 10,000 eukaryotic cells.

Applications

• RT-PCR
• aRNA
• LCM

Benefits

• Quantitatively isolate RNA from 1 or more cells
• Purify RNA for aRNA synthesis
• Simple, nontoxic procedure

Related items:

TargetAmp™ RNA Amplification Kits: these kits can be used to prepare labeled target aRNA (cRNA) for use with virtually any DNA microarray platform including Affymetrix GeneChip^®, GE CodeLink™ or other commercial chips or self-spotted arrays).