The production of PI(3,4,5)P3 from PI(4,5)P2 by type-1 PI 3-kinases (PI3-K) is important in multiple cell signaling pathways.

Nowadays,the measurement of PI 3-K activity involves phosphorylation of a phosphoinositide substrate using radioactive 32P followed by extraction of radioactive products, and separation by chromatography.

Our PIP3 Mass ELISA (Catalog Number K-2500) is a competitive and sensitive (to 1 pmol) ELISA in which the signal is inversely proportional to the amount of PI(3,4,5)P3 produced.

Our PIP3 Mass Strip kit (Catalog Number K-2400) quantifies PIP3 obtained from cell extractions or PI3K reactions through a simle lipid-protein overlay experiment.

Whatever the format you will choose, the PIP3 Mass assays will make your research easier:

• No more need for radioactivity
• No use of organic solvents and thin layer chromatography
• Quick and simple protocols
• Two formats available: Lipid protein overlay (Strip) or ELISA

To know more about our large offer of PI3-K assays in the environment, please click here.