• You don't want to spend time on this,
  
  Use Ready-to-use siRNA against a specific target.
  
  tebu-bio are currently offering over 3,400 target-specific 19-25 nucleotide long siRNAs that can be used to knockdown protein expression in a broad variety of mammalian cell types. Our product line includes siRNAs designed to silence a large selection of proteins including tumor suppressors, kinases, transcription regulators, cell cycle proteins, membrane receptors, kinases, signaling intermediates, cell adhesion proteins and proteins involved in lymphocyte signaling.

To find your target, please use our search engine or send us an e-mail for a help to the search.

Example: Human Cytochrome c siRNA (Cat nr sc-29292): Immunofluorescence staining of methanol fixed control HeLa (A)and cytochrome c siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA treated cells. Cells probed with cytochrome c (7H8) antibody (Cat nr sc-13560).

• You want your own specific design, Make your short hairpin RNA in vitro.
  
  Produce transfection-ready shRNA in 4 hours using a technique easier than other in vitro siRNA construction methods. You don’t need to anneal RNA strands. Using MessageMuter shRNAi production kit allows getting high yields of shRNA.

Overview of the method:



The MessageMuter shRNA Production Kit utilizes a simple and unique 3-step process that yields transfection-ready shRNA in about 4 hours. A 17-base T7 Promoter Oligo is annealed to a DNA oligo designed by the user using detailed instructions provided. The ends of the annealed duplex are "filled-in" using the Exo-Minus Klenow to obtain a linear dsDNA with a T7 RNAP promoter at its 5'-end. Following in vitro transcription, the RNA produced spontaneously forms a hairpin structure (shRNA). Following clean-up, the shRNA is ready for transfection into cultured cells.

The MessageMuter shRNA kit provides reagents to produce 10 shRNAs in sufficient quantities for hundreds of RNAi experiments.

• Not enough time to perform your silencing? We can do it for you in our custom service lab.
  
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