A unique freeze-thaw protocol
Based on a unique freezing-thawing protocol, SeraCare human cryopreserved PBMCs display more than 80% viability.

T cell functionality maintained
The thawed PBMCs maintain full functionality compared to the fresh cells.
Whatever the format you will select (characterized PBMCs or uncharacterized), the frequencies and per-cell cytokine productivities of the thawed cells approximate 100% of the fresh PBMCs when tested for peptide or protein antigen-induced T cell recall responses in cytokine ELISPOT assays.

Cells are from HLA-typed healthy human donors. All donor leukopaks are tested for: HBsAg, HBcAb, HCV, HTLV I/II and STS by serology; as well as HIV I, HCV and WNV by NAT.

Characterized batches
Characterized PBMCs certicate of analysis also includes gender and age information. The PBMCs of each donor have been characterized for reactivity to a panel of 23 individual peptides (common viral Class I-restricted determinants) and 5 protein recall antigens, recognized by CD8 and CD4 cells, respectively. The highly characterized PBMCs may be used to facilitate human immunological research and offer reference samples for assay standardization within laboratories, and between different laboratories.


Technical resources!

Posters

A procedure for cryopreservation of PBMCs that does not alter significantly CD4 and CD8 responses to recall antigens.
Zhang et al., Freeze-Thawing of Multiple Sclerosis Patient's PBMC Does not Impair T Cell Functions as Measured by ELISPOT Assays.

The benefits of the SeraCare's proprietary PBMCs cryopreservation procedure on immunological research programs.
Zhang et al., HLA-Typed PBMC Samples with established Antigen/Peptide Reactivity for Accelerating and Standardizing Human Immunological Research.

Datasheets
An example of datasheet for characterized PBMCs.


An example of datasheet for uncharacterized PBMCs;