For all gene expression studies using qPCR it is necessary to compensate for differences between samples due to the experimental biaises (material losses, differences in RT yields and PCR inhibition...)

Normalization is then used to "compensate" the variations making interpretation of the reults more accurate. It should include an endogenous control gene, but can also be complemented by identical sample input amounts. The endogenous control gene should have constant expression in all the samples compared. There is no universal control gene, expressed at a constant level under all conditions and in all tissues.

The best way to choose the proper reference gene is by running a panel of potential genes on a number of representative test samples. The gene(s) most appropriate for normalization are chosen in each case.

We offer endogenous Control Panel that consists of 12 validated qPCR assays for the most common endogenous control genes for gene expression studies. This endogenous Control Gene Panel (available for Human and Mouse) provides a rapid and cost efficient way to identify your control genes while being compatible with most commercial mastermixes containg SYBR Green I.

One kit contains 100 rxns of 12 genes and is enough for several evaluations of appropriate reference gene.