Microarray Profiling Capabilities with Real-Time PCR Performance!

PCR Arrays are the most reliable tools for analyzing the expression of a focused panel of genes, particularly if you are more familiar with real-time PCR-based techniques than microarray-based methods (SABiosciences, Frederick, MD).

 

• Pathway Focused Design for a thoroughly researched panel of relevant, pathway- or disease-focused genes

• Reliable and Sensitive: Real-time PCR method provides greater sensitivity and a wider linear dynamic range.

• Compatibility with most common machines

• Ease-of-Use: Simply mix your template with the appropriate ready-to-use PCR master mix. Then, aliquot equal volumes to each well of the same plate.

To know more, download the PCR arrays brochure: profile your pathway using qRT-PCR.

Latest list of RT PCR Array available

Select the focused PCR Array tailored to your research projects.

PCR arrays are sold in various convenient pack sizes: 2 arrays, 12 Arrays and 24 Arrays.


If you cannot find the panel of genes you want in our product listing, our custom PCR Array service is quick and affordable. We can make custom PCR Arrays containing any sets of Huamn, Rat or Mouse genes (More details on our custom PCR arrays webpage).

RT PCR Array Buyer’s Guide (plates format and related products)

Find the correct plate format and PCR master mix for the instrument in your laboratory.

PCR Array User Manual

Please contact us to receive the latest version of the manual: europe@tebu-bio.com.

Additional information about the layout of the cataloged pathway-focused PCR Arrays (96-well PCR Array)

Wells A1 through G12 contain primers for genes from the same biological pathway or the same disease state or genes that are otherwise functionally-related.

Wells H1 through H5 contain a housekeeping gene panel to normalize PCR Array data.The product information included with each cataloged PCR Array contains a list of the pathway-focused and housekeeping genes on the array.

Well H6 contains a Genomic DNA Control (GDC) primer set that specifically detects nontranscribed genomic DNA contamination with a high level of sensitivity.

Wells H7 through H9 contain replicate Reverse Transcription Controls (RTC) to test the efficiency of the RT2 PCR Strand Kit (C-03) reaction with a primer set detecting the template synthesized from the kit’s built-in external RNA control.

Wells H10 through H12 contain replicate Positive PCR Controls (PPC) to test the efficiency of the polymerase chain reaction itself using a pre-dispensed artificial DNA sequence and the primer set that detects it.

The two sets of replicate control wells (RTC and PPC) also test for inter-well, intra-plate consistency.

Data Analysis

We offer you to download an easy-to-use Excel-based data analysis template (based on the Ct mathod with normalization of the raw data to either housekeeping genes or an external RNA control).

Just paste in your gene list and Ct values in our Excel template.

Experience the performance by yourself with our Starter Pack OFFER includes

• Two PCR Arrays focusing on the pathway of your choice: Choose from any of our currently available PCR Arrays.

• One RT² First Strand cDNA Synthesis Kit (Cat nr C-03)

• A 200 rxn- Real-Time Master Mix (Buyer's guide)

^{Please contact us to know more: europe@tebu-bio.com.}