The FailSafe™ GREEN Real-Time PCR System (developed by Epicentre Biotechnologies, Madison, CA) uses:

- the FailSafe™ PCR Enzyme Mix

- and an extensively tested set of 12 FailSafe™ GREEN PCR "PreMixes", representing various PCR conditions.
These PreMixes contain everything you need for successful quantitative PCR: SYBR Green I dye, dNTPs, buffer, and varying amounts of MgCl₂ and EPICENTRE's patented FailSafe™ PCR Enhancer with Betaine.

Permits successful real-time PCR and identification of the optimal real-time PCR conditions in one round of PCR.

FIRST TIME Perform real-time PCR with your template and primers using the FailSafe™ GREEN Real-Time PCR Optimization Kit and select the optimal Real-Time PCR PreMix.

→

AND EVERY TIME For consistent PCR results, choose the PCR PreMix identified as optimal when you get the FailSafe™ GREEN Real-Time PCR PreMix-Choice Kit.

Benefits

• Real-Time PCR PreMixes contains all components for quantitative PCR using SYBR Green I dye.
• No specific probes required.
• Includes ROX, a passive reference dye (required for signal normalization in SYBR Green dye reactions when using ABI real-time PCR instruments).
• Kits available for both tube and capillary instruments.

Products List

Cat nr	Size
FailSafe™ GREEN Real-Time PCR Optimization Kit
FSR0360	96 25-µl Reactions
Contents: FailSafe™ PCR Enzyme Mix, 12 FailSafe™ GREEN Real-Time PCR 2X PreMixes, and Passive Reference Dye
FailSafe™ GREEN Real-Time PCR PreMix-Choice Kit
FSR03200	400 25-µl Reactions and choose two PreMixes
FailSafe™ GREEN Real-Time PCR Capillary Optimization Kit
FSRC3832	32 20-µl Reactions
Contents: FailSafe™ PCR Enzyme Mix and 8 FailSafe™ GREEN Real-Time PCR Capillary 2X PreMixes.
FailSafe™ GREEN Real-Time PCR Capillary PreMix-Choice Kit
FSRC3896	96 20-µl Reactions and Choose one PreMix

For Pricing, please use our search engine by indicating the reference you have chosen, at the top of this page or contact us.