Guaranteed Real-Time PCR assays for every gene

You don't need to spend time optimising your primer design for gene-by-gene expression PCR analysis anymore.
You just need to pick up the one you need in our selection of more than 57,000 ready-made gene specific primer sets.

• Whole Genome coverage

RT² specific primers available for every gene in the human, mouse and rat genomes, allowing you to quickly confirm any array results.

• Quick selection with our search engine

Within minutes, you can easily find your ready-made RT² primer sets by using our Primer Sets search engine.
RT² PCR gene-specific primers sets are available in 200 reaction sizes to accomodate different scales of experiment and also suitable for End-Point PCR.

• Guaranteed quality

All our primer pairs are checked using a universal reference RNA to ensure single product amplification. These quality control process guarantees that these primers yield a single band of the predicted size by agarose gel electrophoresis.
RT² Instrument-specific master mixes available for SYBR® Green real-time PCR analysis contains a specially formulated enzyme which prevents amplification of secondary products such as primer dimers.

• Find the master mix optimized for use with your real-time instrument:

The combination of our RT² PCR Primer Sets and our RT² Real-Time™ PCR Master Mixes, both optimized for SYBR Green detection, insures the reliability and sensitivity of the PCR Array. RT² Real-Time PCR MasterMixes are 2X solution with SYBR Green and instrument-specific fluorescent dyes already added.

PCR Master Mix	Catalog Number	Instrument
RT² Real-Time™ SYBR Green/ ROX	PA-012	ABI
RT² Real-Time™ SYBR Green / Fluorescein	PA-011	BioRad iCycler®
RT² Real-Time™ SYBR Green	PA-010	Cepheid SmartCycler® & Others without reference dye requirement
ReactionReady™ HotStart "Sweet"	PA-007	Conventional PCR (No real time instrument available)

• Combine the profiling capabilities with the performance of Real-Time PCR

To assess the mRNA expression of several genes in a single The RT² Profiler™ PCR Arrays is a 96-well plate that includes gene specific primer sets for 84 pathway-focused genes.