Simple and powerful tools for examining signal transduction pathways

- Recombinant small G proteins (wild-type, dominant negative, constitutively active, His- or GST-tagged…)

- Complete assays to monitor in vivo Rac and other small G proteins activation by rapid affinity purification

- GAP or GEF assays

• NEW Quantitative, HTS compatible G-LISA Activation Assay Kits

Measure RhoA, RhoA/B/C, Rac or Cdc42 activation from cell and tissue samples in less than 3 h. G-LISA requires only 1-5% of the material needed for a conventional pulldown assay, and is suitable to get quantitative data for large sample numbers

• User Friendly Pull-Down Activation Assay Kits

No need to look for reagents from different sources any more: Cdc42, Rac1, Ras and RhoA activation assays include all the necessary reagents from lysate precipitation to western blot detection.
• Blue Affinity Beads coated respectively with PAK-PBD, RAF-RBD or Rhotekin RBD, and brightly coloured for easy follow-up of centrifugation efficiency
• no need for specific columns
• Dedicated anti small G-protein specific antibody
• Recombinant G-protein (positive control for western blot)



• GAP and GEF assays without radioactivity

• Determine the GTPase specificity of a GEF or a GAP
• Discover co-factors or small molecules that will modulate GAFs or GAPs

New! A unique cell permeable Rho Inhibitor for live-cell assays!