Arrays
miRNA PCR Arrays
RT² miRNA PCR Arrays enable you to obtain high-quality and genome-wide miRNA expression data by doing nothing more than a simple RT-PCR protocol.

Download the miRNA flyer for further information.

Simultaneous detection of genome-wide or pathway-focused miRNA

The RT² miRNA PCR Array accurately analyzes the expression of multiple micro RNA sequences at the same time on ANY real-time PCR instrument. SABiosciences' patent-pending miRNA technology ingeniously integrates a universal tailing and reverse transcription reaction specific for miRNA with the accurate expression level measurement of distinct miRNA sequences that may only differ by a single nucleotide base. With this technology, you can easily get a comprehensive survey of miRNA expression in your cell line or tissue of interest.

  • Sensitivity: As little as 0.5 µg total RNA needed
  • Multi-Sequence Flexibility: Analyze up to 376 sequences simultaneously
  • Simplicity: As easy as a real-time PCR experiment

Each 96- or 384-well plate contains SYBR Green optimized and experimentally validated assays for either the entire human miRNA genome or pathway-focused sets of human miRNA sequences.

Available miRNA PCR Arrays

Designation Species Cat nr
RT2 Cancer miRNA PCR Arrays Human
Mouse		 MAH-102
MAM-102
RT2 cell differentiation & development miRNA PCR Arrays Human
Mouse		 MAH-103
MAM-103
RT2 miFinder miRNA PCR Arrays Human
Mouse		 MAH-001
MAM-001
Genome RT2 miRNA PCR Arrays (96-well plate) Human
Mouse		 MAH-100A
MAM-100A
Genome RT2 miRNA PCR Arrays (384-well plate) Human
Mouse		 MAH-3100A
MAM-3100A		  

 

Required miRNA PCR Accessories:

* RT2 qPCR-grade miRNA isolation Kit (Cat nr MA-01)
* RT2 miRNA First strand Kit (Cat nr MA-03)

* RT2 qPCR MasterMix w/ SYBR Green/Rox
(Cat nr PA-012)
* RT2 qPCR MasterMix w/ SYBR Green/Fluorescein
(Cat nr PA-011)
* RT2 qPCR MasterMix w/ SYBR Green only
(Cat nr PA-010)



Single nucleotide mismatch discrimination

RT² miRNA Assay Design Improves Discrimination & Specificity. The proprietary primer design of the RT² miRNA PCR Array and Assays distinguishes miRNA family members with single nucleotide mismatches providing greater discrimination and specificity than other commercial providers.


Synthetic
miRNA Template		 Assay Primers Relative Detection
(%Perfect Match)		 miRNA Template Sequence
miR-10a miR-10a 100.00 UACCCUGUAGAUCCGAAUUUGUG
miR-10b 0.98
miR-10b miR-10b 100.00 UACCCUGUAGAACCGAAUUUGUG
miR-10a 0.01
miR-99a mir-99a 100.00 AACCCGUAGAUCCGAUCUUGUG
miR-100 0.54
miR-100 miR-100 100.00 AACCCGUAGAUCCGAACUUGUG
mir-99a 0.07
miR-196a miR-196a 100.00 UAGGUAGUUUCAUGUUGUUGGG
miR-196b 0.20
miR-196b miR-196b 100.00 UAGGUAGUUUCCUGUUGUUGGG
miR-196a 1.64

Relative detection as a percent of the perfect match (100 x 2- ΔΔCt) is calculated from the Ct values of target and off-target assays used to detect 105 copies of synthetic miRNA template. The observed cross-reactivity seen between RT² miRNA qPCR Assays for different miRNA species are compared.

 

Distinction between mature miRNA and their precursors

Specifically detecting mature miRNA improves sensitivity. The patent-pending chemistry of the RT² miRNA First Strand Kit preferentially reverse transcribes mature miRNA, thereby decreasing non-specific background and increasing sensitivity. The RT² miRNA PCR Assays provide three orders of magnitude greater sensitivity than competing assays. Other assays can amplify non-specific products that tend to be more evident at lower amounts of input material.

Synthetic mir-658 was serially diluted into a constant amount (100 ng) of small RNA enriched from 293H cells lacking that sequence. Samples were analyzed with mir-658-specific RT² miRNA qPCR Assays and other commercial assays. The resulting threshold cycles of the assays are plotted versus the log10 of the number of mir-658 template copies.

 



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