Animal Primary Cells
Rat Osteoblasts (ROb)

 

Rat Osteoblasts (ROb) are isolated from rat bone and are cryopreserved at second passage. ROb can be passaged at least 10 population doublings. ROb are a powerful tissue engineering tool for the study of signaling pathways and 3-D modeling. Sustained delivery of basic fibroblast growth factor (bFGF) enhances bone formation following osteoblast transplantation1. Prostaglandin E increases fibronectin and promotes bone formation in ROb via the EP(1) receptor, phospholipase C, PKCalpha, and c-SRC signaling pathway2. Via the FGF2 pathway, prostaglandin F2-alpha regulates ROb metabolism3. Osteoblasts express A disintegrin and metalloprotease-1 (ADAMTS-1), which breaks down extracellular matrix molecules and is expressed at regions of new bone formation4. ADAMTS-1 is a target for parathyroid hormone in bone5. Hypoxia decreases the growth, differentiation and bone-forming capacity of ROb6. ROb can be used in tissue engineering models. Rat osteoblasts have been cultured in 3-D matrices, including processed bovine cancellous bone which resulted in osteoblast differentiation7. Plasma-treated porous polylactide membranes increases the attachment and growth of ROb for the use of tissue engineering8.  

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
ROb Total Kit Each kit contains an ampoule of cryopreserved ROb (R406-05), 500 ml of Rat Osteoblast Growth Medium (R417-500), and a Subculture Reagent Kit (090K). 1 kit 095R406K-05
 
Cryopreserved ROb (>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095R406-05
 
Rat Osteoblast Growth Medium Optimized and ready for use. 500 ml 095R417-500
 
Rat Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095R417K-500
 
Rat Osteoblast Basal Medium Add Growth Supplements before use. 500 ml 095R416-500
 
Rat Osteoblast Growth Supplements   50 ml 095R417-GS
 
Rat Osteoblast Differentiation Medium   250 ml 095R417D-250
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090-K
 
  1. Park, MS et al., J Biomec Mater Res B Appl Biomater 79(2):353 (2006).
  2. Tang, CH et al., J Biol Chem 280(24):22907 (2005).
  3. Marchetti, L et al., J Cell Biochem 97(6):1379 (2006).
  4. Lind, T e tl., Bone. 36(3):408 (2005).
  5. Miles, RR et et al., Endocrinology. 141(12):4533 (2000).
  6. Utting, JC et al., Exp Cell Res. 312(10):1693 (2006).
  7. Stangenberg, L et al., Tissue Eng. 11(5-6):855 (2005).
  8. Gugala, Z & Gogolewski, S, J Biomed Mater Res A. 76(2):288 (2006).
Would you like to have more information?
Contact us