Animal Primary Cells
Rat Neural Stem Cells (RNSC)
Rat Neural Stem Cells (RNSC) are isolated from cerebral striatum and mesencephalon of E18 fetal rat brains. These cells were maintained in undifferentiated proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors. They are cryopreserved at second passage and can be cultured and propagated for 1-2 passages1 prior to induction of phenotypic differentiation. These stem cells are multipotent2,3 and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS). Neurospheres transplanted into intact brain can survive, expand and differentiate into mature neurons, astrocytes and oligodendrocytes precursors4 in 3 weeks. The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries.  

Rat Neural Stem Cells

Rat Neurosphere

Rat Neural Stem Cells

Differentiated RNSC stained for a-Tubulin

Characterization: Differentiated neural stem cells are positive for a-tubulin III, GFAP and oligodendrocyte marker O4.

Bioassay: Each lot is tested for its ability to form neurospheres in Rat Neural Stem Cell Growth Medium.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.

RNSC Total Kit,

adult (a)

fetal (f)

Each kit contains an ampoule of cryopreserved RNSC (RS820-20a or RS820-20f), 250 ml of Rat Neural Stem Cell Growth Medium (R813-250).

 1 Kit 095RS820K-20a

095RS820K-20f

Cryopreserved RNSC

adult (a)

fetal (f)

 (>500,000 cells in an ampoule) Cryopreserved at first passage in Rat Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095RS820-20a

095RS820-20f
Rat Neural Stem Cell Growth Medium

Optimized and ready for use

 250 ml 095R813-250
Rat Neural Stem Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 250 ml 095R813K-250
Rat Neural Stem Cell Basal Medium Add Growth Supplement before use 250 ml 095R812-250
Rat Neural Stem Cell Growth Supplement   10 ml 095R813-GS
Rat Neural Stem Cell Differentiation Medium   250 ml 095R813D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
  1. Svendsen, C.N. et al, Brain Res. Dev. 99:253 (1997).
  2. Reynolds, B.A. et al, J. Neuroscience 12(11):4565 (1992).
  3. Reynolds, B.A. et al, Develop. Bio. 175:1 (1996).
  4. Karbanova, J. et al. Biomed. Papers 148(2):217 (2004).
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