Animal Primary Cells
Rat Lung Fibroblasts (RLF)

 

Rat Lung Fibroblasts (RLF) are derived from the lung of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RLF can be cultured and propagated 10 population doublings. It was found that TGF-beta and PDGF produced by stimulated macrophages are involved in the regulation of the expression of alpha1(1) collagen, of prolyl-4-hydroxylase and lysyloxidase in rat lung fibroblasts1. Antisense calcyclin (S100A6) RNA expression was found to inhibit serum and mechanical strain induced fibroblasts proleferation2. Activation of fibroblasts by TGF-beta(1) by interferon-gamma may have potential therapeutic benefits in bleomycin-induced pulmonary fibrosis3. Furthermore, sulfated proteoglycans play a key role in modulating pulmonary fibroblast extracellular matrix composition and are important mediators of elastolytic injury4, and addition of heparin-binding EGF-like growth factor to pulmonary fibroblasts increases FGF-2 mRNA and protein levels.    
Characterization: Positive for fibroblast surface protein.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

  
Product Name Product Description Size (vol)

Cat. No.
RLF Total Kit

Each kit contains an ampoule of cryopreserved RLF (R506-05), 500 ml of Rat Fibroblast Growth Medium (R116-500), a Subculture Reagent Kit (090K).

 1 Kit 095R506K-05
 
Cryopreserved RLF (>500,000 cells in an ampoule) Cryopreserved at second passage in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

 095R506-05
 
Rat Fibroblast Growth Medium

Optimized and ready for use

 500 ml 095R116-500
 
Rat Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095R116K-500
 
Rat Fibroblast Basal Medium Add Growth Supplement before use 500 ml 095R115-500
 
Rat Fibroblast Growth Supplement   50 ml 095R116-GS
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution) 100 ml 095090-K
 
  1. Koslowski, R. et al, Exp. Toxicol Patholo. 2003 Nove; 55(4): 257-64.
  2. Breen EC, Tang K, J Cell Biochem, 2003 Mar 1; 88(4): 848-54
  3. Venkatesan, N. et al Am J Physiol Lung Cell Mol Physiol. 2002 Octµ 283(4): L806-14
  4. Buczek-Thomas JA, et al, J Cell Physiol. 2002 Sep; 192(3): 294-303
  5. Liu, J, et al, Am J Physiol Lung Cell Mol Physiolo. 2003 Nov; 285(5):L1106-15
 


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