Animal Primary Cells
Rat Dermal Fibroblasts (RDF)

Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings.

The arginase activity was significantly elevated in woundµderived fibroblasts from Lewis rats compared to normal dermal fibroblasts underlines the distinct regulation of l-arginine metabolism in wound-derived fibroblasts1. RDF were modified genetically to act like Schwannµs cells to deliver nerve growth factor2. The role of skin fibroblast elastase on the degeneration of elastic fiber caused by UV irradiation was tested on the rat hind leg3.

   Characterization: Positive for fibroblast surface protein expression.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.
RDF Total Kit

Each kit contains an ampoule of cryopreserved RDF (R106-05), 500 ml of RDF Growth Medium (R116-500), and a Subculture Reagent Kit (090K).

 1 Kit 095R106K-05
 
Cryopreserved RDF (>500,000 cells in an ampoule) Cryopreserved at first passage in RDF Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095R106-05
 
RDF Growth Medium

Optimized and ready for use.

 500 ml 095R116-500
 
RDF Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095R116K-500
 
RDF Basal Medium Add Growth Supplement before use 500 ml 095R115-500
 
RDF Growth Supplement   50 ml 095R116-GS
 
Subculture Reagent Kit

(100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution)

 100 ml 095090-K
 
 
  1. Witte, M.B. et al, J. Surg. Res. 105(1):35-42 (2002).
  2. Patrick, C.W. et al, Ann. Plast. Surg. 47(6):660-5 (2001).
  3. Tsukahara, K. et al, J. Invest. Dermatol. 117(3):671-7 (2001).
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