Animal Primary Cells
Rat Cardiomyocytes (RCm)

 

Rat cardiomyocytes (RCm) are primary cells derived from normal rat heart tissue and are plated in inserts or plates immediately after primary isolation. C-type natriuretic peptide (CNP) is found to have a proapoptotic effect on neonatal RCm while endothelin-1 protects RCm against CNP-induced apoptosis1. Coculturing skeletal muscle cells isolated from transgenic mice with RCm showed that some part of the skeletal muscle cells can transdifferentiate into cardiomyocytes and that direct cell-to-cell contact and contraction of neighboring cardiomyocytes are important for the transdifferentiation2. Cardiac myofibrils extracted from freshly isolated adult rat ventricular myocytes were used to assess the actomyosin adenosine triphosphatase activity and phosphorylation of troponin I (TnI) and myosin light chain 2 (MLC2)3. Growth hormone was found to prolong the survival of rats with post infarction heart failure. This effect was associated with the attenuation of cardiomyocytes apoptosis in the surviving myocardium and enhanced left ventricle relaxation and pathologic interstitial remodeling4. A review of the transverse tubule function in mammalian cardiac myocytes is available5.  

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.
Rat Cardiomyocyte Growth Medium

Optimized and ready for use

 500 ml 095R313-500
 
Rat Cardiomyocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095R313K-500
 
Rat Cardiomyocyte Basal Medium Add Growth Supplement before use 500 ml 095R312-500
 
Rat Cardiomyocyte Growth Supplements   80 ml 095R313-GS
 
 
  1. Han, B. et al, Eur. J. Pharmacol. 474(1): 15-20 (2003).
  2. Iijima, Y. et al. FASEB J. 17(10): 1361-3(2003).
  3. Kanaya N, et al. Anesthesiology, 98(6):1363-71 (2003).
  4. Cittadini, A. et al, J. Am. Coll. Cardiol. 41(12):2154-63 (2003).
  5. Brette, F. and Orchard C. Circ. Res. 92(11):1182-92 (2003).
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