Animal Primary Cells
Rat Aortic Smooth Muscle Cells (RAOSMC)
Rat Aortic Smooth Muscle Cells (RAOSMC) are derived from the tunica intima and tunica media of healthy, fibrous plaque-free rat aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Increased arterial smooth muscle cell mass are found in the intimal lesion of atherosclerosis1. Rat aortic smooth muscle cells respond to various factors by cell proliferation2 and hypertrophy3, which are prominent indicators of atherosclerosis in vascular diseases. RAOSMC are well suited for the study of large vessel smooth muscle cell growth and differentiation4,5 and serve as in vitro model in correlation with live rat models.  

Characterization: Positive for smooth muscle cell specific a - actin expression.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.
RAOSMC Total Kit

Each kit contains an ampoule of cryopreserved RAOSMC (R354-05), 500 ml of Rat SMC Growth Medium (R311-500), and a Subculture Reagent Kit (090K).

1 Kit

 095R354K-05
 
Cryopreserved RAOSMC (>500,000 cells in an ampoule) Cryopreserved at second passage in Rat SMC Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

 095R354-05
 
Rat SMC Growth Medium

Optimized and ready for use

 500 ml 095R311-500
 
Rat SMC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095R311K-500
 
Rat SMC Basal Medium Add Growth Supplement before use 500 ml 095R310-500
 
Rat SMC Growth Supplement   50 ml 095R311-GS
 
Rat SMC Differentiation Medium   250 ml 095R311D-250
 
Subculture Reagent Kit

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution

 100 ml 095090K
 
RAOSMC RNA

Total RNA prepared from RAOSMC

 10 µg 25 µg 095R354-R10

095R354-R25
 
 
  1. Ross, R., Nature 362:801 (1993).
  2. Heldin, C.H. et al, Mol. Cell. Endocrinol. 39:169 (1985).
  3. Berk, B.C. et al, J. Biol. Chem. 265:17334 (1990).
  4. Owen, G.K. et al, J. Cell Biol. 102:343 (1986).
  5. Skalli, O. et al, J. Cell Biol. 103(6):2787 (1986).
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