Animal Primary Cells
Rat Brain Microvascular Endothelial Cells (RBMVEC)

Rat Brain Microvascular Endothelial Cells (RBMVEC) are derived from the brain of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RBMVEC can be cultured and propagated 16 population doublings. HIV-1 Tat protein alters the tight junction protein expression and distribution in cultured brain endothelial cells. It is believed such alteration lead to disturbances of the blood-brain barrier (BBB) integrity and contributes to HIV trafficking in to the brain1. Endothelial cell-derived monocyte-chemoattractant protein-1 (MCP-1) plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo2 . Three-dimensional model indicates that dipyridamole can improve BBB Function3. Mainteance of BBB function during and following normoxic-cormoglycemic flow cessation appears to depend on intact nitric oxide signaling and IL-6 release4. Expression of metabotropic glutamate receptors may modulate synaptic transmission and determine microvascular function and dysfunction5. VEGF mRNA and VEGF receptor F1K-1/KDR are both higher in neonatal than in adult microvessels suggesting that microvascular cell turnover continues in the adult brain6. Hypoxia increases the aparacellualr flux across the cell monolayer via the release of VEGF, which in turn leads to the dislocalization, decreased expression and enhanced phosphorylation of Zonula occludens-1(ZO-1)7.

 

 Rat Brain Microvascular Endothelial Cells
Tissue obtained from USDA inspected animals.  
Product Name Product Description Size (vol)

Cat. No.
RBMVEC Total Kit Each kit contains an ampoule of cryopreserved RBMVEC (R840-05), 500 ml of Rat Brain Endothelial Cell Growth Medium (R819-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K). 1 Kit R840K-05
Cryopreserved RBMVEC (>500,000 cells in an ampoule) Cryopreserved at second passage in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule R840-05
RBMVEC Growth Medium

Optimized and ready for use.

 500 ml R819-500
RBMVEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml R819K-500
RBMVEC Basal Medium Add Growth Supplement before use. 500 ml R818-500
RBMVEC Growth Supplement   50 ml x 2 R819-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090-K
RBMVEC RNA Total RNA prepared from RBMVEC

10 µg

25 µg

R840-R10

R840-R25
 
  1. Andras IE, et al, J Neurosci Res. 2003 Oct 15; 74(2): 255-65
  2. Harkness, KA, et al, Neuroimmunol. 2003 Sep; 142(1-2): 1-9
  3. Parkinson FE, et al, Brain Res. 2003 Aug. 8; 980(2) 233-41
  4. Krizanac-Bengez, L, et al, Brain Res. 2003 Jul. 11; 977(2): 239-46
  5. Gillard, SE, et al, J Comp. Neurol. 2003 Jun. 30; 461(3): 317-32
  6. Hoehn, BD, et al, Brain Res. Mol Brain Res. 2002 May 30; 101(1-2): 103-8
  7. Fischer S. et al, Microvasc Res. 2002 Jan; 63(1): 70-80

 

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