Animal Primary Cells
Rat Aortic Endothelial Cells (RAOEC)

Rat Aortic Endothelial Cells (RAOEC) are isolated from aorta of healthy adult rats. They are cryopreserved at third passage and can be cultured and propagated to sixth passage and beyond without losing their morphologic and phenotypic characteristics. RAOEC is a relevant in vitro model for correlating with in vivo studies in rat as an animal model. RAOEC have been used as a model for the study of Atherosclerosis1 and products synthesized by the endothelium2,3. The interaction of aortic endothelium and smooth muscle cells regulate the level and distribution of glycosaminoglycans in the arterial wall, especially in the intima4. Co-culture of aortic endothelial cells and smooth muscle cells provide an ideal in vitro model for studying the interaction between these two cells types.

  

DiI-Ac-LDL Uptake

Characterization: Positive for DiI-Ac-LDL uptake.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.
RAOEC Total Kit

Each kit contains an ampoule of cryopreserved RAOEC (R304-05), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit.

 1 Kit 095R304K-05
Cryopreserved RAOEC (>500,000 cells in an ampoule) Cryopreserved at third passage in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095R304-05
Rat Endothelial Cell Growth Medium

Optimized and ready for use

 500 ml 095R211-500
Rat Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095R211K-500
Rat Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 095R210-500
Rat Endothelial Cell Growth Supplement   15 ml 095R211-GS
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 095123-100
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
RAOEC RNA

Total RNA prepared from RAOEC

10 µg

25 µg

 095R304-R10

095R304-R25
 
  1. Ross, R., Nature 362:801 (1993)
  2. Falcy, C., et. al., Collagen Rel. Res. 5:519 (1985)
  3. McGuire, P.G., et.al., Microcirc.Endo.Lymph. 2:129 (1985
  4. Merrilees, M.J., et.al., Atherosclerosis 39:147 (1981)
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