Animal Primary Cells
Porcine Coronary Artery Smooth Muscle Cells (PCASMC)

Porcine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous plaque-free porcine arteries. They are cryopreserved at first passage (PCASMC at second passage) and can be cultured and propagated at least 16 population doublings (PCASMC at least 14 doublings). Smooth muscle cells have been found in fatty streaks of early arteriosclerosis. Proliferation of the smooth muscle cells is considered a key event in the development of advanced lesions1. Porcine smooth muscle cells serve as an alternative for the investigations of cardiac disease and arteriosclerosis1-3. It can be used in co-culture investigation with species-matched porcine artery endothelial cells.

   Porcine Artery Smooth Muscle Cells

Characterization: Smooth muscle cell specific a -actin expression.

  

Animal tissues were USDA inspected.

  
Product Name Product Description Size (vol)

Cat. No.

Porcine SMC Total Kit

PCASMC

PPASMC

PAOSMC

Each kit contains an ampoule of cryopreserved Porcine Smooth Muscle Cells, 500 ml Porcine SMC Growth Medium, and a Subculture Reagent Kit (090K).

 1 Kit 095P350K-05

095P352K-05

095P354K-05

Cryopreserved Porcine SMC

PCASMC

PPASMC

PAOSMC

 (>500,000 cells in an ampoule) Cryopreserved at first passage (PCASMC at second passage) in Porcine SMC Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095P350-05

095P352-05

095P354-05
Porcine SMC Growth Medium Optimized and ready for use 500 ml 095P311-500
Porcine SMC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095P311K-500
Porcine SMC Basal Medium Add Growth Supplement before use 500 ml 095P310-500
Porcine SMC Growth Supplement   50 ml 095P311-GS
Porcine SMC Differentiation Medium   250 ml 095P311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
  1. Ross, R.N., Nature 362:801 (1993).
  2. Stary, H.C., Arteriosclerosis 3:471a (1983).
  3. McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).
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