Animal Primary Cells
Porcine Pulmonary Artery Endothelial Cells (PPAEC)

Porcine large artery endothelial cells are derived from healthly, plaque free porcine. PAOEC and PPAEC are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. PCAEC is cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. Arterial endothelial cells play critical roles in cardiac homeostasis. Insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells have been used as a viable alternative for the study of artery endothelial function and metabolism, such as atherosclerosis1, relaxing factor synthesis2, antioxidant enzyme activities3, etc. Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.

  

Characterization: Positive for Dil-Ac-LDL uptake.

  

Animal tissues were USDA inspected.

  
Product Name Product Description Size (vol)

Cat. No.

Porcine EC Total Kit

PCAEC

PPAEC

PAOEC

Each kit contains an ampoule of cryopreserved Porcine Endothelial Cells, 500 ml of Porcine Endothelial Cell Growth Medium (P211-500), and a Subculture Reagent Kit (090K).

 1 Kit 095P300K-05

095P302K-05

095P304K-05

Cryopreserved Porcine EC

PCAEC

PPAEC

PAOEC

 (>500,000 cells in an ampoule) Cryopreserved at first passage (PCAEC at second passage) in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095P300-05

095P302-05

095P304-05
Porcine EC Growth Medium Optimized and ready for use 500 ml 095P211-500
Porcine EC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095P211K-500
Porcine EC Basal Medium Add Growth Supplement before use 500 ml 095P210-500
Porcine EC Growth Supplement   50 ml 095P211-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K

Porcine EC RNA

PPAEC

PAOEC

Total RNA prepared from Porcine Endothelial Cells

 10 µg 095P302-R10

095P304-R10

Porcine EC RNA

PPAEC

PAOEC

Total RNA prepared from Porcine Endothelial Cells

 25 µg 095P302-R25

095P304-R25
 
  1. Ross, R.N., Nature 362:801 (1993).
  2. Boulanger, C.M. et al, J. fo Vasc. Res. 34(3):175 (1997).
  3. Bishop, C.T. et al, In Vitro Cell Devel. Biol. 21:229 (1985).
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