Animal Primary Cells
Mouse Neural Stem Cells (MNSC)

Mouse Neural Stem Cells (MNSC) are isolated from cerebral striatum and mesencephalon of E14 fetal mouse brains. These cells were maintained in an undifferentiated proliferative state by culturing them as free-floating neurospheres in serum-free medium optimized with growth factors. MNSC are cryopreserved at second passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. These stem cells are multipotent1,2 and can be induced to differentiate into neurons, astrocytes and oligodendrocytes. Neural stem cells are a source of donor tissue for prospective neural cell therapy given the ability of neurosphere to retain multi-lineage potential and proliferative ability in vitro.

  

MNSC

Differentiated MNSC stained for a-Tubulin

Characterization: Differentiated mouse neural stem cells are positive for a-tubulin III, GFAP and oligodendrocyte marker O4.

Bioassay: Each lot is tested for its ability to form neurospheres in Mouse Neural Stem Cell Growth Medium.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.
Product Name Product Description Size (vol)

Cat. No.
MNSC Total Kit, fetal (f) Each kit contains an ampoule of cryopreserved MNSC (MS820-20f), 250 ml of Mouse Neural Stem Cell Growth Medium (M813-250). 1 kit 095MS820K-20f
Cryopreserved MNSCf (>500,000 cells in an ampoule) Cryopreserved at first passage in Mouse Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095MS820-20f
MNSC Growth Medium

Optimized and ready for use .

 250 ml 095M813-250
MNSC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 250 ml 095M813K-250
MNSC Basal Medium Add Growth Supplement before use 250 ml 095M812-250
MNSC Growth Supplement   10 ml 095M813-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
  1. Reynolds, B.A. et al, J. Neuroscience 12(11):4565 (1992).
  2. Reynolds, B.A. et al, Develop. Bio. 175:1 (1996).
 
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