Animal Primary Cells
Canine Skeletal Muscle Cells (CnSkMC)
Canine Skeletal Muscle Cells (CnSkMC) are derived from the limbal skeletal muscle and are cryopreserved at second passage. CnSkMC can be passaged at least 15 population doublings. Muscle diseases can occur in dogswith clinical presentations resembling human disorders, thus the caninesystem can be a valuable intermediate disease model1,2. The canine model has been used to study the effects of hypoxia3,4,5, ischemia6,7, and hemorrhagic shock8 on skeletal muscle. CnSkMC represent an in vitro cellular system to study the effects of diet on skeletal muscle9,10,11.  

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
CnSkMC Total Kit Each kit contains an ampoule of cryopreserved CnSkMC (Cn150-05), 500 ml of Canine Skeletal Muscle Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K). 1 kit 095Cn150K-05
Cryopreserved CnSkMC (>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095Cn150-05
Canine Skeletal Muscle Cell Growth Medium Optimized and ready for use. 500 ml 095Cn151-500
Canine Skeletal Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095Cn151K-500
Canine Skeletal Muscle Cell Basal Medium Add Growth Supplements before use. 500 ml 095Cn150-500
Canine Skeletal Muscle Cell Growth Supplements   2 x 50 ml 095Cn151-GS
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090-K
  1. Shelton, GD & Enqvall, E, Neuromuscul Disord. 15(2):127 (2005).
  2. Ytterberg, SR, Curr Opin Rheumatol. 3(6):934 (1991).
  3. Mo, FM & Ballard, HJ. Am J Physiol Heart Circ Physiol 289(4):H1710 (2005).
  4. Mo, FM & Ballard, HJ. J Physiol. 536(2):592 (2001).
  5. da Cruz, CA et al., J Cardiovasc Surg (Torino). 38(5):473 (1997).
  6. Timmons, JA et al., J Clin Invest. 97(3):879 (1996).
  7. Roberts, PA et al., J Physiol. 544(2):591 (2002).
  8. Sunder-Plassman, L et al., Res Exp Med (Berl) 159(3):167 (1973).
  9. McKenzie, E et al., Med Sci Sports Exerc. 37(8(:1307 (2005).
  10. Helman, EE et al., J Anim Sci. 81(9):2199 (2003).
  11. Reynolds, Aj et al., J Appl Physiol. 79(5):1601 (1995).
 
Would you like to have more information?
Contact us