Animal Primary Cells
Canine Osteoblasts (CnOb)
Canine Osteoblasts (CnOb) are isolated from canine bone and are cryopreserved at second passage. CnOb can be passaged at least 10 population doublings. The dog model provides a system for the study of bone remodeling agents such as osteoblast-stimulating bone morphogenetic proteins (BMPs)1,2 and bisphosphonates3, as well as anti-remodeling agents4. Canine osteoblasts have been used in prospective bone regeneration models utilzing such 3-D bioactive scaffolds as polycaprolactone5.  

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
CnOb Total Kit Each kit contains an ampoule of cryopreserved CnOb (Cn406-05), 500 ml of Canine Osteoblast Growth Medium (Cn417-500), and a Subculture Reagent Kit (090K). 1 kit 095Cn406K-05
Cryopreserved CnOb (>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Osteoblast Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095Cn406-05
Canine Osteoblast Growth Medium Optimized and ready for use. 500 ml 095Cn417-500
Canine Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095Cn417K-500
Canine Osteoblast Basal Medium Add Growth Supplements before use. 500 ml 095Cn416-500
Canine Osteoblast Growth Supplements   30 ml 095Cn417-GS
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090-K
  1. Schmoekel, HG et al., J Orthop Res. 22(2):376 (2004).
  2. ten Dijke, P. Curr Med Res Opin. 22(S):S7 (2006).
  3. Im, GI et al., Biomaterials. 25(18):4105 (2004).
  4. Allen, MR et al., Calcif Tissue Int. 79(4):255 (2006).
  5. Rai, B et al., Biomaterials. 25(24):5499 (2004).
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