Animal Primary Cells
Canine Aortic Smooth Muscle Cells (CnAOSMC)

 

Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings. Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in intimal hyperplasia and atherosclerosis. It was shown that significant regional difference in PDGF production in normal canine aorta, and that SMC are a significant contributor to the regional variation in PDGF production1. It was also shown that nebivolol relaxes vascular smooth muscle by NO- and cyclic GMP-dependent mechanisms2. Graft SMCs are functionally altered producing more platelet-derived growth factor (PDGF) than aortic SMCs. PDGF produced by graft SMCs may contribute to the development of intimal hyperplasia3. Graft SMCs have decreased proliferation response, but have similar migratory response to PDGF compared with aortic SMCs4. Intrinsic differences in endothelial cells from proximal aorta versus the distal aorta have different capacity to produce PDGF in response to stimulants whereas unstimulated SMCs did not exhibit regional variation in PDGF production and did not increase PDGF secretion after PMA or thrombin teatment5. Identification of the cDNA for canine isoform of nitric oxide synthase (iNOS) can be used in the study of allograft rejection and cardiovascular disease6.   Animal Cells - Canine Aortic Smooth Muscle Cells Characterization: Positive for smooth muscle specific a-actin expression.  
 
Product Name Product Description Size (vol) Cat. No.
CnAOSMC Total Kit Each kit contains an ampoule of cryopreserved CnAOSMC (Cn354-05), 500 ml of Canine SMC Growth Medium (Cn311-500), and a Subculture Reagent Kit. 1 Kit 095Cn354K-05
Cryopreserved CnAOSMC (>500,000 cells in an ampoule) Cryopreserved at second passage in Canine SMC Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095Cn354-05
Canine SMC Growth Medium

Optimized and ready for use

 500 ml 095Cn311-500
Canine SMC Growth Medium Kit

Basal Medium & Growth Supplement packaged separately

 Yields 500 ml 095Cn311K-500
Canine SMC Basal Medium Add Growth Supplement before use 500 ml 095Cn310-500
Canine SMC Growth Supplement   50 ml 095Cn311-GS
Canine SMC Differentiation Medium   250 ml 095Cn311D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
  1. Ignarro, L.J. et al, Nitric Oxide. 7(2):75-82 (2002).
  2. Madura, J.A. et al, J. Vas Res. 33(1):53-61 (1996).
  3. Pitsch, R.J. et al, J. Vasc. Surg. 26(1):70-8 (1997).
  4. Minion, D.J. et al, J. Vasc. Surg. 31(5):953-9 (2000).
  5. Van Aalst, J.A. et al, J. Vasc. Surg. 32(3):584-92 (2000).
  6. Wang, X. et al, Am. J. Physiol. 275(4 Pt 2):H1122-9 (1998).
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