Animal Primary Cells
Canine Aortic Endothelial Cells (CnAOEC)

 

Canine Aortic Endothelial Cells (CnAOEC) are endothelial cells isolated from the dog aorta. CnAOEC are cryopreserved at second passage and can be passaged at least 16 doublings. The dog model provides an in vitro system to study cardiovascular diseases such as aortic aneurysms1. The aorta of the canine is commonly used as a model for stent-graft testing for the aneurysm treatment2,3,4. Studies have shown that cultured canine aortic endothelial cells express cardiotrophin-1 (CT-1), glycoprotein (gp)130, and the vasoconstricting and mitogenic peptide endothelin-1 (ET-1)5. Cultured CnAOEC have also been shown to release vasodilatory products in response to acetylcholine and adenosine6.  

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
CnAOEC Total Kit Each kit contains an ampoule of cryopreserved CnAOEC (Cn304-05), 500 ml of Canine Endothelial Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K). 1 kit 095Cn304K-05
Cryopreserved CnAOEC (>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095Cn304-05
Canine Endothelial Cell Growth Medium Optimized and ready for use. 500 ml 095Cn211-500
Canine Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095Cn211K-500
Canine Endothelial Cell Basal Medium Add Growth Supplements before use. 500 ml 095Cn210-500
Canine Endothelial Cell Growth Supplements   30 ml 095Cn211-GS
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090-K

  1. Weon, YC et al., AJR Am J Roentgenol 186(4):1148 (2006).
  2. Castaneda, F et al., Cardiovasc Intervent Radiol. 23(5):375 (2000).
  3. Konya, A & Wright, KC. Acta Radiol. 41(1):67 (2000).
  4. Jougasaki, M et al., Peptides. 23(8):1441 (2002).
  5. Woodley, N & Barclay, JK. Can J Physiol Pharmacol 74(8):949 (1996).

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