Animal Primary Cells
Bovine Endometrial Epithelial Cells (BEnEpC)
 

Bovine Endometrial Epithelial Cells (BEnEpC) are isolated from epithelium of the bovine endometrium. The cells are cryopreserved at second passage. BEnEpC can be cultured and propagated for at least 10 population doublings. BEnEpC are commonly used with Bovine Endometrial Stromal Cells as an in vitro model for studying maternal recognition of pregnancy, such as the effects of IFN-t and oxytocin,1,2,3 the effects of prostaglandins on the maintenance, or lack thereof, of pregnancy,3,4 and the effects of estradiol and progesterone.5 In addition, it has been shown that BEnEpC can maintain embryo development to blastocyte stage as effectively as oviductal cells.6

 

Characterization: Positive for DiI-Ac-LDL uptake.

Animal tissues were USDA inspected.
Product Name Product Description Size (vol)

Cat. No.

BEnEpC Total Kit

Each kit contains an ampoule of cryopreserved BEnEpC (B932-05), 500 ml of BEnEpC Growth Medium (B911-500), a Subculture Reagent Kit (090K).

1 Kit 095B932K-05
Cryopreserved BEnEpC (>500,000 cells in an ampoule) Cryopreserved at second passage in Bovine Endometrial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095B932-05
BEnEpC Growth Medium

Optimized and ready for use

500 ml 095B911-500
BEnEpC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 095B911K-500
BEnEpC Basal Medium Add Growth Supplement before use 500 ml 095B910-500
BEnEpC Growth Supplement   50 ml x 2 095B911-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
  1. Desnoyers, G.D. et al, Reprod. Fertil. Dev. 6:193-202 (1994)
  2. Meyer, M.D. et al, J. Dairy Sci. 79:1375-1384 (1996)
  3. Horn, S. et al, Hum. Reprod. Update. 4(5):605-614 (1998)
  4. Asselin, E. & Fortier, M.A., J. Cell Physiol. 168(3):600-607 (1996)
  5. Xiao, C.W. et al, Endocrinology 139(5):2293-2299 (1998)
  6. Goff, A.K. & Smith, L.C., Theriogenalogy 49(5):1021-1030 (1998)
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