Animal Primary Cells
Bovine Brain Microvascular Endothelial Cells (BBMVEC)
Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings. Brain Microvascular Endothelial Cells form the basis of the blood-brain barrier, and have been used as a model for research on bacterial meningitis1,2,3. Hypoxia-ischemia studies showed that BBMVEC cell death may exacerbate ischemic brain injury4. BBMVEC have also been used to show oxyhemoglobin induces apoptosis in vascular endothelial cells5. Co-culture with Atrocytes generate an in vitro model that closely mimics the in vivo situation for evaluating drug transport6.   Animal Cells - Bovine Brain Microvascular Endothelial Cells

Characterization: Positive for DiI-Ac-LDL uptake.

  

Animal tissues were USDA inspected.

  
Product Name Product Description Size (vol)

Cat. No.
BBMVEC Total Kit

Each kit contains an ampoule of cryopreserved BBMVEC (B840-05), 500 ml of BBMVEC Growth Medium (B811-500), and a Subculture Reagent Kit (090K).

 1 Kit 095B840K-05
Cryopreserved BBMVEC (>500,000 cells in an ampoule) Cryopreserved at second passage in Bovine Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095B840-05
BBMVEC Growth Medium

Optimized and ready for use

 500 ml 095B819-500
BBMVEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095B819K-500
BBMVEC Basal Medium Add Growth Supplement before use 500 ml 095B818-500
BBMVEC Growth Supplement   50 ml x 2 095B819-GS
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution) 100 ml 095090-K
BBMVEC RNA

Total RNA prepared from BBMVEC

10 µg

25 µg

 095B840-R10

095B840-R25
 
  1. Zysk, G. et al, Infect. Immun. 69(2):845-852 (2001)
  2. Prasadarao, N.V. et al, Infect. Immun. 67(3):1131-1138(1999)
  3. Wang, Y. et al, Infect. Immun. 67(9):4751-4756 (1999)
  4. Zhang, J. et al, Brain Res. 877(2):134-140 (2000)
  5. Meguro, T. et al, J. Neurochem. 77(4):1128-1135
  6. Cecchelli, R. et al, Adv. Drug Deliv Rev 36:165 (1999)
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