Human Primary Cells
Human Umbilical Vein Smooth Muscle Cells (HUVSMC)
Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings. Human umbilical vein actively regulates its vascular tone under the control of pO2. Vasodilation endothelial prostanoids contribute to the hypoxic vasodilation that counteracts the vasoconstricting actions of prostanoids released from HUVSMC. It was found that vasoconstricting prostanoids are released from the endothelium of the human umbilical vein at hyperoxia1. During preeclampsia a total loss of neuronal nitric oxide synthase (nNOS) protein expression and a significant decrease in mRNA expression were seen. It was found that such loss might alter the regulation of blood flow in the fetal and maternal placental vasculature2. Both ouabain and marinobufagenin activate proliferation of the HUVSMC. The concentration ranges of both compounds were found to be below that necessary to induce cytoplasmic ion alterations by sodium pump inhibition3.   Umbilical Vein Smooth Muscle Cells, HUVSMC
 

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HUVSMC Total Kit, neonatal

Each kit contains an ampoule of cryopreserved HUVSMC (250-05n), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.

1 Kit

 095250K-05n
 

Cryopreserved HUVSMC , neonatal

(>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

 095250-05n
 

Smooth Muscle Cell Growth Medium

Optimized and ready for use .

500 ml

 095311-500
 
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation   250 ml 095311D-250
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
 

  1. Mildenberger E, et al. J. Perinat. Med. 2004;32(2):149-54.

  2. Schonfelder G, et al. Histopathology. 2004 Feb; 44(2):116-28.

  3. Abramowitz J, et al. Circulation. 2003 Dec 16; 108(24):3048-53.

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