Human Primary Cells
Human Umbilical Artery Smooth Muscle Cells (HUASMC)

Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Umbilical artery smooth muscle cells have been used as an economical alternative for the study of morphological and structural changes in the development of atherosclerosis1,2.

  

Human Umbilical Artery Smooth Muscle Cells

Characterization: Positive for smooth muscle cell specific a -actin expression.
Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
Product Name Product Description Size (vol)

Cat. No.
HUASMC Total Kit, neonatal

Each kit contains an ampoule of cryopreserved HUASMC (252-05n), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).

1 Kit

 095252K-05n
 
Cryopreserved HUASMC, neonatal (>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

 095252-05n
 
Smooth Muscle Cell Growth Medium

Optimized and ready for use.

 500 ml 095311-500
 
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation Medium   250 ml 095311D-250
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
HUASMC RNA , neonatal

Total RNA prepared from HUASMC.

10 µg

25 µg

 095252-R10n

095252-R25n
 
 

  1. Ross, R.N., Nature 362:801 (1993).

  2. McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).

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