Human Primary Cells
Human Umbilical Artery Endothelial Cells (HUAEC)

Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. Arterial endothelial cells have been used as an in vitro model for the study of morphological and ultrastructural changes in the development of atherosclerosis1. The HUAEC system has also been used for the study of endothelial cell-dependent lymphocyte activation .and chemoattractant activity2,3.

  

Characterization: Positive for Factor VIII-related antigen expression. Positive for Factor VIII-related Dil-Ac-LDL uptake.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
Product Name Product Description Size (vol)

Cat. No.
HUAEC Total Kit, neonatal

Each kit contains an ampoule of cryopreserved HUAEC (202-05n), 500 ml of HUAEC Growth Medium (212-500), and a Subculture Reagent Kit.

 1 Kit 095202K-05n
 
Cryopreserved HUAEC, neonatal (>500,000 cells in an ampoule) Cryopreserved at first passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095202-05n
 
HUAEC Growth Medium

Optimized and ready for use .

 500 ml 095212-500
 
HUAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095212K-500
 
HUAEC Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
HUAEC Growth Supplement   30 ml 095212-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 
HUAEC RNA, neonatal

Total RNA prepared from HUAEC

10 µg

25 µg

 095202-R10n

095202-R25n
 
 
  1. Antonor, A.S. et al, Atherosclerosis 59:1 (1986).
  2. Ashida, E.R. et al, J. Clin. Inv. 67:1490 (1981).
  3. Farber, H.W. et al, Amer. J. Physiol. 262 (4 parts):H1088 (1992).
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