Human Primary Cells
Human Marrow Stromal Cells (HMSC)

 

 
Human Marrow Stromal Cells (HMSC) are isolated from human bone marrow. They are cryopreserved at second passage and can be cultured and propagated 10 population doublings. In addition to supporting hematopoietic cells1, marrow stromal cells can be induced to differentiate into cells of different connective tissue lineage, such as bone, cartilage, and fat2,3,4. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue5,6. Recent reports indicate that HMSCs are capable of cell fates crossing germ layer boundaries. In addition to differentiating into multi-lineages of the mesoderm, these cells can also differentiate into neurons7 of ectodermal origin and hepatocyte-like cells8 of endodermal origin.   HMSC von Kossa Stain

Characterization: Differentiated marrow stromal cells are positive for bone mineralization and lipid accumulation.

Bioassay: Each lot is tested for its ability to attach and spread on standard tissue culture grade plastic surfaces and proliferate in Marrow Stromal Cell Growth Medium

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
HMSC Total Kit Each kit contains an ampoule of cryopreserved HMSC (492-05f), 500 ml of Human Marrow Stromal Cell Growth Medium (419-500), and a Subculture Reagent Kit (090K). 1 kit 095492K-05f
 
Cryopreserved HMSC, fetal (>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095492-05f
 
Human Marrow Stromal Cell Growth Medium Optimized and ready for use. 500 ml 095419-500
 
Human Marrow Stromal Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095419K-500
 
Human Marrow Stromal Cell Basal Medium Add Growth Supplements before use. 500 ml 095418-500
 
Human Marrow Stromal Cell Growth Supplements   30 ml 095419-GS
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090-K
 
  1. Verfaillie, C.M., Blood 82:1045 (1993).
  2. Bruder, S.P. et al, J. Cell. Biochem. 64:278 (1997).
  3. Mackay, A..M. et al, Tissue Engineering 4:415 (1998).
  4. Pittenger, M.F. et al, Science 284:143 (1999).
  5. Horwitz, E.M. et al, Blood 92 Suppl 1:249 (1998).
  6. Bruder, S.P. et al, J. Orthop. Res. 16:155 (1998).
  7. Woodbury, D., et al, J. Neurosci. Res. 61:364 (2000).
  8. Oh, S.H., et al, Biochem. Biophy. Res. Commun. 279:500 (2000).
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