Human Primary Cells
Human Epidermal Keratinocytes (HEK)

Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products1, and for developing dermal skin substitutes2. HEK has been used in numerous applications, including investigations of human epidermal development3, differentiation4, and cellular aspects of skin diseases5.

   human epidermal keratinocytes, hek HEK human epidermal keratinocytes, cytokeratin peptide stained hek HEK stained for cytokeratin peptide 14
Characterization: Positive for Cytokeratin Peptide 14.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
Product Name Product Description Size (vol)

Cat. No.

HEK Total Kit

adult

fetal

neonatal

Each kit contains an ampoule of cryopreserved HEK (102-05), 500 ml of HEK Cell Growth Medium (131-500) and a Subculture Reagent Kit.

 1 Kit 095102K-05a

095102K-05f

095102K-05n
 

Cryopreserved HEK

adult

fetal

neonatal

 (>500,000 cells in an ampoule) Cryopreserved at the end of primary culture in Keratinocyte Basal Medium that contains 30% FBS and 10% DMSO. 1 Ampoule 095102-05a

095102-05f

095102-05n
 
HEK Growth Medium

Optimized and ready for use

 500 ml 095131-500
 
HEK Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095131K-500
 
HEK Basal Medium Add Growth Supplement before use 500 ml 095130-500
 
HEK Growth Supplement   5 ml 095131-GS
 
HEK Calcium-Free Growth Medium   500 ml 095133-500
 
HEK Calcium-Free Growth Medium Kit   Yields 500 ml 095133K-500
 
HEK Calcium-Free Basal Medium   500 ml 095132-500
 
HEK Calcium-Free Growth Supplement   5 ml 095133-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 

HEK RNA

adult

neonatal

Total RNA prepared from HEK

10 µg

25 µg

10 µg

25 µg

 095102-R10a

095102-R25a

095102-R10n

095102-R25n
 
 
  1. Delco, V. et al, Alternative Methods in Toxicology, 3: 466 (1985).
  2. Joyce, S.T. et al, J. Biomed. Mater. Res. 22:937 (1988).
  3. Holbrook, K.A., Biochemistry and Physiology of the Skin, Vol. 1:64 (1983).
  4. Fuchs, F.J., Cell Biol. 111 (No. 6, Pt. 2):2807 (1990).
  5. Koizami, H. et al, British J. Dermatol. 134: 686 (1996).
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