Human Primary Cells
Human Dermal Fibroblasts (HDF)

Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. Fibroblasts are found in all connective tissues, and they synthesize and secrete extracellular matrix proteins under cell culture conditions1. They are a well established system for in vitro analysis of fibroblast growth, migration and collagen metabolism2-5 in wound healing. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement6,7.

    

Characterization: Positive for fibroblast surface protein.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name

Product Description

Size (vol)

Cat. No.
HDF Total Kit
adult(a)
fetal(f)
neonatal(n)

Each kit contains an ampoule of cryopreserved HDF (106-05), 500 ml of Fibroblast Growth Medium (116-500), and a Subculture Reagent Kit (090K).

 1 Kit 095106K-05a

095106K-05f

095106K-05n
Cryopreserved HDF
adult
fetal
neonatal		 (>500,000 cells in an ampoule) Cryopreserved at the end of primary culture in Fibroblast Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095106-05a

095106-05f

095106-05n
Fibroblast Growth Medium

Optimized and ready for use

 500 ml 095116-500
Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095116K-500
Fibroblast Basal Medium Add Growth Supplement before use 500 ml 095115-500
Fibroblast Growth Supplement   15 ml 095116-GS
Subculture Reagent Kit 100ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
HDF RNA fetal neonatal

Total RNA prepared from HDF

10 µg

25 µg

10 µg

25 µg

 095106-R10f

095106-R25f

095106-R10n

095106-R25n
 
  1. Hedman, K. et al, J. Cell Biol. 81:83 (1979).
  2. Gay, S. et al, Proc. Natl. Acad. Sci. 73:4037 (1976).
  3. Booth, A.B. et al, Biochem. Biophys. Acta 607:145 (1980).
  4. Peterkofsky, B. et al, Proc. Natl. Acad. Sci. 53:335 (1965).
  5. Hausmann, E., Biochem. Biophys. Acta 133:591 (1967).
  6. Hansborough, J.F. et al, Surgery 111(4):438 (1991).
  7. Cooper, M.L. et al, Biomaterials 12:243 (1991).
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