Human Primary Cells
Human Placental Epithelial Cells (HPlEpC)

Human Placental Epithelial Cells ( HPlEpC ) are isolated from normal human amnion. They are cryopreserved at the end of primary culture. These epithelial cells proliferate well when they are thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization.

HPlEpC has been used to study the secretion of activin A1, the neural formation during early fetal development2, and the antimicrobial and endotoxin-neutralizing barrier effect of Histones H2A and H2B against microorganism3. Prostaglandin E2 induces high level of amniotic epithelial cell death via necrosis rather than apoptosis4. Matrix metalloproteinasesµ9 (MMP-9) localized mainly in amnion epithelia. An increase in MMP-9 expression may contribute to degradation of extracellular matrix in fetal membrane and placenta, thereby facilitate fetal membrane rupture and placental detachment5.

  

Human Placental Epithelial Cells

Characterization: Typical epithelial cell morphology.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size

Cat. No.

HPlEpC Total Kit

fetal

neonatal

Each kit contains an ampoule of cryopreserved HPlEpC (230-05), 500 ml of Placental Epithelial Cell Growth Medium (215-500), and a Subculture Reagent Kit (090K).

 1 Kit 095230K-05f

095230K-05n
 

Cryopreserved HPlEpC

fetal

neonatal

 (>500,000 cells in an ampoule) Cryopreserved at primary passage in Placental Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095230-05f

095230-05n
 
Placental Epithelial Cell Growth Medium

Optimized and ready for use

 500 ml 095215-500
 
Placental Epithelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095215K-500
 
Placental Epithelial Cell Basal Medium Add Growth Supplement before use 500 ml 095214-500
 
Placental Epithelial Cell Growth Supplement   5 ml 095215-GS
 
Subculture Reagent Kit 100ml each of HBSS, Trypsin/EDTA & Neutralizing Solution 100ml 095090-K
 

HPlEpC RNA

fetal

neonatal

Total RNA prepared from HPlEpC

 10 µg 095230-R10f

095230-R10n
 

HPlEpC RNA

fetal

neonatal

Total RNA prepared from HPlEpC

 25 µg 095230-R25f

095230-R25n
 
 
  1. Schneider-Kolsky, M.E., et al, Placenta 23(4):294-302 (2002).
  2. Koyano, S. et al, Dev. Growth Differ.44(2):103-12 (2002).
  3. Kim, H.S, et al, J. Immunol. 168(5):2356-64 (2002).
  4. Surendran, S. Inflamm. Res. 50(10):483-5 (2001).
  5. Xu, P. et al, J. Clin. Endocrinol. Metab. 87(3):1353-61 (2002).
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