Human Primary Cells
Human Dermal Microvascular Endothelial Cells (HMVEC/CADMEC)

Cell Applications' Dermal Microvascular Endothelial Cells (CADMECµ) are isolated from normal human neonatal foreskin or adult skin capillaries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They are responsive to cytokine stimulation in the expression of cell adhesion molecules. CADMECµ express CD36 constitutively, while large vessel endothelial cells do not. CADMECµ is an organ-specific endothelial cell system for the study of angiogenesis1, tumor metastasis2,3, wound healing4, inflammation5,6, macromolecule transport7, and drug metabolism8. There is growing evidence of differences in physiology and pharmacology between microvascular endothelial cells and macrovascular endothelial cells9,10.

   Human Dermal Microvascular Endothelial Cells HMVEC, CADMEC HMVEC/CADMEC Human Dermal Microvascular Endothelial Cells HMVEC/CADMEC Cytokine Stimulated Leukocyte adhesion.  
Characterization:
  • Positive for Factor VIII-related antigen expression. Positive for Factor VIII-related Dil-Ac-LDL uptake. Positive for CD36 expression.
  • Positive for functional activity by cytokine-stimulated leukocyte adherence.
  
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

We have special lots that were verified to be responsive to VEGF. Please call to discuss how these lots can meet your needs. The activation of VEGF-R2 was validated by autophosphorylation at tyrosine 1175. VEGF-R2 is a high affinity receptor for VEGF whcih stimulates proliferation, chemotaxis and sprouting of endothelial cells in vitro and angiogenesis in vivo . via VEGF-R2 signalling pathway.

click here for Pre-Screened HUVEC and CADMEC Cells...

  
Product Name Product Description Size (vol)

Cat. No.

CADMECµ Total Kit

adult

neonatal

Each kit contains an ampoule of cryopreserved CADMECµ (100-05), 500 ml of CADMECµ Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100).

 1 Kit 095100K-05a

095100K-05n
 

Cryopreserved CADMEC

adult

neonatal

 (>500,000 cells in an ampoule) Cryopreserved at second passage in CADMECµ Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095100-05a

095100-05n
 
CADMEC Growth Medium

Optimized and ready for use

 500 ml 095112-500
 
CADMEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095112K-500
 
CADMEC Basal Medium Add Growth Supplement before use 500 ml 095110-500
 
CADMEC Growth Supplement   30 ml 095112-GS
 
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 095123-100
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090-K
 

CADMEC RNA

adult (a)

neonatal (n)

Stimulated CADMEC Total RNA

adult (a)

neonatal (n)

 Total RNA prepared from CADMECµ Total RNA prepared from stimulated CADMECµ

10 µg

25 µg

10 µg

25 µg

10 µg

25 µg

10 µg

25 µg

 095100-R10a

095100-R25a

095100-R10n

095100-R25n

095100S-R10a

095100S-R25a

095100S-R10n

095100S-25n
 
 
  1. Swerlick, R.A. et.al, J. Invest. Dermatol. 97(2):190 (1991)
  2. Folkman, J. et al, Nature 288:551 (1980).
  3. Lee, K.H. et al, J. Invest. Dermatol. 98(1):79 (1992).
  4. Deryugina, E.I. et al, Hybridoma 15(4):279 (1996)
  5. Sepp, N.T. et al, J. Invest. Dermatol. 104(2):277 (1995).
  6. Swerlick, R.A. et al, J. Invest. Dermatol. 100(1):111S (1993).
  7. Hanasaki, K. et al, J. Bio. Chem. 270(13):7533 (1995)
  8. Pruckler, J.M. et al, Pathobiology 61:283 (1993).
  9. Foster, C.A. et al, J. Dermatol. 21(11):847 (1994).
  10. Schnitzer, J.E. et al, BBRC. 199(1):11 (1994).
Would you like to have more information?
Contact us